GSM2035717: EB AM580-treated day12 on OP9, experiment 2 (q7 in the EB... - SRA

SRX1526332: GSM2035717: EB AM580-treated day12 on OP9, experiment 2 (q7 in the EB_AM580gene_exp.diff); Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 25.2M spots, 1.3G bases, 569.2Mb downloads

Submitted by: NCBI (GEO)

Study: RNA-seq expression data from EB-HSPC after AM580 treatment compated to DMSO-trated and FL-HSPCs

PRJNA308426 • SRP068279 • All experiments • All runs

show Abstracthide Abstract

RA signalling regulated endothelial to hematopoietic transition and HSC generation. Overall design: EB- or FL-derived HSPC were profiled before (d0) or after (d6) 6 days of treatment with 0.2uM AM580 on OP9, and after 6 additional days of expandion of OP9 (d12) without treatment.

Sample: EB AM580-treated day12 on OP9, experiment 2 (q7 in the EB_AM580gene_exp.diff)

SAMN04395224 • SRS1244235 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was purified from sorted cells using RNeasy Mini Kit (QIAGEN), according to manufacturer's instructions. Ovation Rna-seq system System v2 (Nugen), followed by KAPA LTP Library Preparation Kit

Experiment attributes:

GEO Accession: GSM2035717

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 25.2M spots, 1.3G bases, 569.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR3095984	25,226,078	1.3G	569.2Mb	2016-05-17

ID:: 2157603

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